Column Control DTX

Simultaneous AFM/Fluorescence Imaging of Living Cells – Fluorescence-guided Force Spectroscopy

Application Notes

Introduction

 

Atomic force microscopy has evolved as a method capable of resolving biological structures at the molecular level [2]. Furthermore, it provides the possibility to study interaction forces between single receptor-ligand complexes. Fluorescence microscopy has proven to be a powerful method for the specific visualization of labeled molecules down to the single molecule level [1]. This allows the determination of their distribution observing diffusion or trafficking processes in cells. Other optical techniques, such as bright field, phase contrast [3], and DIC microscopy reveal information about cell morphology and composition. By enabling a more detailed characterization of internal and external cellular structures and biological processes, the combination of AFM with optical microscopy provides a more comprehensive examination of the sample than either of the techniques alone can deliver

×

Please have a salesperson contact me.

*Indicates required field

Preferred method of communication? *Required Field
Preferred method of communication? Change email?
Preferred method of communication?

By clicking the button, you are providing Keysight with your personal data. See the Keysight Privacy Statement for information on how we use this data.

Thank you.

A sales representative will contact you soon.

Column Control DTX