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Application Notes
Introduction
Atomic force microscopy has evolved as a method capable of resolving biological structures at the molecular level [2]. Furthermore, it provides the possibility to study interaction forces between single receptor-ligand complexes. Fluorescence microscopy has proven to be a powerful method for the specific visualization of labeled molecules down to the single molecule level [1]. This allows the determination of their distribution observing diffusion or trafficking processes in cells. Other optical techniques, such as bright field, phase contrast [3], and DIC microscopy reveal information about cell morphology and composition. By enabling a more detailed characterization of internal and external cellular structures and biological processes, the combination of AFM with optical microscopy provides a more comprehensive examination of the sample than either of the techniques alone can deliver
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